| Wyatt Langford | 1st | Texas Rangers | 2023 | Trenton | Rangers 3/28/2024 |
| LeVon Washington | 2nd | Cleveland Indians | 2010 | Buchholz | |
| Max White | 2nd | Colorado Rockies | 2012 | Williston | |
| Tucker Neuhaus | 2nd | Milwaukee Brewers | 2013 | Wharton | |
| Garrett Cave | 4th | San Francisco Giants | 2017 | South Sumter | |
| Derrick Robinson | 4th | Kansas City Royals | 2006 | P.K. Yonge | Reds 4/5/2013 |
| Sam McMillan | 5th | Detorit Tigers | 2017 | Suwannee | |
| Jordon Austin | 6th | Boston Red Sox | 2013 | Ocala Forest | |
| Tyler Holt | 10th | Cleveland Indians | 2010 | Gainesville | Indians 7/6/2014 |
| Phoenix Sanders | 10th | Tampa Bay Rays | 2017 | Buchholz | Rays 4/14/2022 |
| Will Allen | 13th | Detorit Tigers | 2014 | Buchholz | |
| Brian Ellington | 16th | Miami Marlins | 2012 | Oak Hall | Marlins 8/3/2015 |
| Josh Falk | 17th | Oakland Athletics | 2017 | Bishop McLaughlin | |
| Trent Johnson | 16th | New York Mets | 2016 | Williston | |
| Eric Grabe | 24th | Chicago White Sox | 2012 | Ocala Vanguard | |
| Kevin McCoy | 24th | Seattle Mariners | 2013 | Gainesville | |
| David Lee | 27th | Pittsburgh Pirates | 2017 | Buchholz | |
| Jack Charleston | 30th | Chicago White Sox | 2015 | Buchholz | |
| Andrew Eyster | 32nd | Cleveland Indians | 2018 | Ocala Forest | |
| Christian Morris | 33rd | New York Yankees | 2015 | Williston | |
| Malcolm Clapsaddle | 34th | Baltimore Orioles | 2009 | Oviedo | |
| Josh Greene | 39th | Chicago Cubs | 2013 | Ocala Forest | |
| Justin Cummings | 39th | Philadelphia Phillies | 2010 | P.K. Yonge | |
| Jacinto Arredondo | FA | Boston Red Sox | 2020 | South Sumter | |
| Gerald Bautista | FA | Cleveland Indians | 2012 | Steinbrenner | |
